Digitally reinforced hematoxylin-eosin polarization technique in diagnosis of rectal amyloidosis

Figure 3 Amyloid suspicious deposits in muscularis mucosa and submucosa (stars). A: Brightfield view of hematoxylin-eosin (HE) staining; B: Digitally reinforced polarization view of the same HE-stained section. Note the nonspecific refractions throughout the tissue and the aberrant artificial refraction of a foreign body on the slide just below the (stars) in A and B. No convincing birefringence for amyloidosis is observed in the suspected area (stars) by HE; C: Brightfield view of Congo-red staining with salmon pink deposits (stars); D: Polarization view of the same Congo-red-stained section with apple green birefringence of deposits (stars); 10× magnification.