Digitally reinforced hematoxylin-eosin polarization technique in diagnosis of rectal amyloidosis

Figure 2 Tiny amyloid deposits in vessel walls, which are not striking at first sight (long arrows). A: Brightfield view of hematoxylin-eosin (HE) staining; B: Digitally reinforced polarization view of the same HE-stained section. Note the striking yellowish green tint of birefringence of amyloid in small vessel walls (long arrows) and variant colored nonspecific refraction of a foreign body (short arrow); C: Brightfield view of Congo-red staining (note the salmon pink color); D: Polarization view of the same Congo-red-stained section with apple green birefringence of deposits (arrows); 20× magnification.