Overexpression of B7-H3 augments anti-apoptosis of colorectal cancer cells by Jak2-STAT3

Figure 3 Overexpression of B7-H3 suppresses apoptosis in colorectal cancer cells by weakening the sensitivity to drugs. Stably transfected SW620 and HCT8 cell lines were incubated with a high concentration of 5-Fu or L-OHP (50 μg/mL) for 48 h. A: The ratio of apoptosis was detected by cell cycle assay according to sub-G1 peak; B: Statistical results were used to analyze the rate of apoptosis from (A); C: Western blot analysis for active Caspase-3 and GAPDH protein levels in whole-cell lysates from stably transfected SW620 and HCT8 cell lines. ^aP < 0.05, ^bP < 0.01 vs control. 5-Fu: 5-Fluorouracil; L-OHP: Oxaliplatin.