Overexpression of B7-H3 augments anti-apoptosis of colorectal cancer cells by Jak2-STAT3

Figure 2 Overexpression of B7-H3 increases cell survival. Stably transfected SW620 and HCT8 cell lines were incubated with 5-Fu or L-OHP for 48 h. A CCK-8 assay was used to detect the inhibition rate of cells treated with different concentration of different drugs. A: The control cells (SW620-NC) and the B7-H3 overexpressing cells (SW620-B7-H3-EGFP) were incubated with 5-Fu; B: The control cells (HCT8-NC) and the B7-H3 knockdown cells (HCT8-shB7-H3) were incubated with 5-Fu; C: SW620-NC and SW620-B7-H3-EGFP were incubated with L-OHP; D: HCT8-NC and HCT8-shB7-H3 were incubated with L-OHP. ^aP < 0.05 vs control. 5-Fu: 5-Fluorouracil; L-OHP: Oxaliplatin; NC: Negative control.