Overexpression of B7-H3 augments anti-apoptosis of colorectal cancer cells by Jak2-STAT3

Figure 1 Overexpression of B7-H3 inhibits apoptosis. A: Real-time PCR for RNA levels of B7-H3, Bcl-2, Bcl-xl and Bax relative to β-actin in stably transfected SW620 cells, control cells (SW620-NC) and B7-H3 overexpressing cells (SW620-B7-H3-EGFP); B: Western blot analysis for B7-H3, Bcl-2, Bcl-xl, Bax and GAPDH protein levels in whole-cell lysates from the SW620 cells; C: Comparison of relative protein levels between the SW620 cells from (B); D: Real-time PCR for RNA levels of B7-H3, Bcl-2, Bcl-xl and Bax relative to β-actin in stably transfected HCT8 cell lines, the control cells (HCT8-NC) and the B7-H3 knockdown cells (HCT8-shB7-H3); E: Western blot analysis for B7-H3, Bcl-2, Bcl-xl, Bax and GAPDH protein levels in whole-cell lysates from the HCT8 cells; F: Comparison of relative protein levels between the HCT8 cells from (E). ^aP < 0.05, ^bP < 0.01 vs control. Bax: Bcl-2-associated X protein; Bcl-2: B-cell CLL/lymphoma 2; Bcl-xl: B-cell lymphoma-extra large; NC: Negative control.