In vitro identification of nonalcoholic fatty liver disease-related protein hnRNPM

Figure 4 Effects of glyceraldehyde modification of heterogeneous nuclear ribonucleoprotein M by high fructose exposure. Cells were incubated with 0.5-10.0 mmol/L fructose for 5 d. A: Cell lysates were separated on two-dimensional gradient sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and the glyceraldehyde modification of heterogeneous nuclear ribonucleoprotein M (hnRNPM) was determined by probing with anti-Glycer-AGEs antibody. The arrow shows spots identified with hnRNPM; B: Cell lysates were separated by SDS-PAGE and probed with anti-hnRNPM antibody. Equal protein loading was determined using anti-β-actin antibody; C: The levels of mRNA expression were analyzed by real-time RT-PCR, and the result was normalized to β-actin. Data are shown as mean ± SD (n = 6); ^aP < 0.05 vs control. Left panel: With 2 mmol/L fructose, right panel: With 10 mmol/L fructose.