In vitro identification of nonalcoholic fatty liver disease-related protein hnRNPM

Figure 1 Western blot analysis of intracellular glyceraldehyde-derived advanced glycation end-products. Cells were incubated with or without 2 mmol/L fructose for 5 d. Cell lysates (100 μg of protein/gel) were separated on two-dimensional gradient sodium dodecyl sulfate-polyacrylamide electrophoresis and probed with Anti-glyceraldehyde-derived advanced glycation end-products (Glycer-AGEs) antibody (A, B), or anti-Glycer-AGEs antibody neutralized by an excess of Glycer-AGEs-bovine serum albumin (C, D); A,C: control samples; B,D: Fructose samples. The arrow shows spots where a difference was seen.