CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan

Figure 5 Western blotting analysis of natriuretic peptide receptor B expression in rat rectum. A: Natriuretic peptide receptor B (NPR-B) and β-actin protein expression levels in rat rectum tissue. Assayed NPR-B protein or β-actin by immunoblot analysis. β-actin was used as a loading control. Lane N: Primary control group; Lane M: Depression model group; Lane D: Low Xiaoyaosan (XYS) dose group; Lane Z: Middle XYS dose group; Lane G: High XYS dose group. Protein bands have been altered via image processing software (Adobe Photoshop CS3) to get a cleaner background; B: Panel shows the expression of NPR-B and β-actin protein levels by relative optical density measurement in rat rectum. Data are presented as mean ± SE. The difference between the depression model group and the primary control group was significant (n = 9; ^bP < 0.01 vs M). The high and middle XYS dose groups were significantly different compared with the depression model group (n = 9; ^bP < 0.01 vs M). CNP: C-type natriuretic peptide.