CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan

Figure 4 Real-time polymerase chain reaction analysis of C-type natriuretic peptide in rat rectum. A: C-type natriuretic peptide (CNP) mRNA transcript levels were normalized to β-actin. B: CNP mRNA transcript levels were normalized to GAPDH. Values of ΔΔCt are expressed as ratios of model group expression level. β-actin and GAPDH are used as the internal control. Bars represent data presented as mean ± SE. N: Primary control group; M: Depression model group; D: Low dose Xiaoyaosan (XYS) group; Z: Middle dose XYS group; G: High dose XYS group. The difference between the depression model group and the primary control group was significant (n = 9; ^bP < 0.01 vs M). The high and middle XYS dose groups were significantly different compared with the depression model group (n = 9; ^bP < 0.01 vs M).