CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan

Figure 2 Open-field test of the five groups of rats. A: Number of grids crossed: (instances that three or more paws crossed into adjacent grids); B: Standing number (number of times two forelimbs were lifted up from the bottom of box to make the body upright); C: Grooming times (number of times two forelimbs were used for grooming, scratching, or licking face and paws). Data are presented as mean ± SE. N: Primary control group; M: Depression model group; D: Low dose Xiaoyaosan (XYS) group; Z: Middle dose XYS group; G: High dose XYS group. On the 21^st day, the difference between the depression model group and the primary control group was significant (n = 9; ^bP < 0.01 vs M). The difference between the XYS groups and the depression model group was significant (n = 9; ^bP < 0.01 vs M).