S-phase arrest after vincristine treatment may promote hepatitis B virus replication

Figure 3 Vincristine promotes hepatitis B virus protein expression in HepG2-HBV1. 1 and HepG2.2.15 cells. A: Vincristine up-regulates HBeAg expression. HBeAg in the supernatant was analyzed using an ELISA assay; B: Vincristine increases cellular HBcAg expression based on Western blot analysis; C: The effect of vincristine on intracellular HBsAg expression was analyzed using an ELISA assay; D: The effect of vincristine on HBsAg expression in the supernatant was analyzed using an ELISA assay. HepG2-HBV1.1 (6 × 10⁵/well) and HepG2.2.15 (4 × 10⁵/well) cells were treated with vincristine at a concentration of 0.1 μmol/L for 24 h followed by 48 h of incubation in drug-free culture medium. Values represent the mean ± SD of 3 independent experiments. ^aP < 0.05, ^bP < 0.01 vs control group (no vincristine). HBeAg: Hepatitis B e Antigen; HBcAg: Hepatitis B c Antigen; HBsAg: Hepatitis B surface antigen.