S-phase arrest after vincristine treatment may promote hepatitis B virus replication

Figure 1 Effect of vincristine on the viability of stable hepatitis B virus-expressing cell lines. A: The effect of vincristine on the viability of HepG2.2.15 and HepG2-HBV1.1 cells based on the trypan blue exclusive assay; B: The effect of vincristine on the viability of HepG2.2.15 and HepG2-HBV1.1 cells based on the MTT assay. HepG2.2.15 (4 × 10⁵/well) and HepG2-HBV1.1 (6 × 10⁵/well) cells were treated with vincristine at various concentrations (0-0.5 μmol/L) for 24 h, followed by 48 h of incubation in drug-free culture medium. The cells were collected 72 h after drug treatment. The results are expressed as the arithmetic mean ± SD from 3 independent experiments.