MiR-21/RASA1 axis affects malignancy of colon cancer cells via RAS pathways

Figure 3 MiR-21 negatively regulates RAS p21 GTPase activating protein 1. A: Luciferase reporter gene assay showed lower RLU values in pre-miR-21-LV cells co-transfected with pGL3-promoter-LucifeRASe-RASA1 in comparison with cells co-transfected with pGL3-promoter-LucifeRASe (n = 3, ^bP < 0.01); B: qRT-PCR detection of RASA1 mRNA levels in cells with up/down-regulated miR-21 (n = 3, ^aP < 0.05 vs control; ^cP < 0.05 vs control); C: Western blot was used to assess RASA1 protein levels in cells with up/down-regulated miR-21. 1: anti-miR-21-LV; 2: anti-NC-LV; 3: control; 4: pre-miR-21-LV; 5: pre-NC-LV. RASA1: RAS p21 GTPase activating protein 1; NC: Non-coding; LV: Lentivirus.