MiR-21/RASA1 axis affects malignancy of colon cancer cells via RAS pathways

Figure 2 Validation of transfection efficiency. A: The expression of miR-21 in cells with up/down-regulated miR-21 was assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (n = 4, ^aP < 0.05 vs control; ^cP < 0.05 vs control); B: qRT-PCR was used to quantify the expression of RASA1 mRNA (n = 3,^aP < 0.05 vs control; ^cP < 0.05 vs control); C: Western blot detection of RASA1 protein levels; 1: Control; 2: pcDNA3.1-RASA1 NC; 3: pcDNA3.1-RASA1; 4: siRASA1 NC; 5: siRASA1. RASA1: RAS p21 GTPase activating protein 1; NC: Non-coding; LV: Lentivirus.