Xiaotan Sanjie decoction inhibits interleukin-8-induced metastatic potency in gastric cancer

Figure 5 Effects of Xiaotan Sanjie decoction and interleukin-8 on the expression of CD44 mRNA in SGC-7901 cells (quantitative PCR). A: Marker DL2000 (from top to bottom: 1000, 750, 500, 250, 100 bp); B-E: hGAPDH (blank group, IL-8 group, XTSJ group, XTSJ + IL-8 group, 145 bp); F-I: CD44 (blank group, IL-8 group, XTSJ group, XTSJ + IL-8 group, 230 bp). CD44 mRNA expression was determined by quantitative PCR. Similar to protein expression, CD44 mRNA expression was significantly up-regulated by IL-8, but was not influenced by XTSJ decoction. Importantly, XTSJ decoction down-regulated the CD44 mRNA expression induced by IL-8. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.