Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats

Figure 6 Intestinal zona occludens-1 immunofluorescent staining at 6 h after blood loss. In the sham group, zona occludens (ZO)-1 presented with strong fluorescent intensity. Rats in the hemorrhagic shock (HS) group showed low fluorescent intensity at the cell periphery after HS, and electroacupuncture (EA) ST36 showed preservation of the robust structure of ZO-1 staining, whereas EA ST36 after vagotomy (VGX) or injection of α-bungarotoxin (BGT) eliminated such protection. All images were taken at magnification × 1000 magnification; white bar = 10 μm. (n = 3-6 rats per group at 6 h after blood loss, section size = 2 μm). A: Sham group; B: HS group; C: EA group; D: VGX/EA group; E: VGX group; F: α-BGT/EA; G: α-BGT group.