Hypoxia-inducible factor-1 modulates upregulation of mutT homolog-1 in colorectal cancer

Figure 2 Hypoxic stress-induced mutT homolog-1 expression in colon carcinoma cell lines. SW480 and HT-29 cells were incubated under normal or hypoxic conditions. mRNA relative expression of MTH-1 was evaluated by qRT-PCR, as shown in A and C (48 h: P = 0.013, P = 0.041; 72 h: P = 0.000, P = 0.000). Protein levels of MTH-1 were evaluated by western blotting, as shown in B and D. ^aP < 0.05, ^bP < 0.01, normoxia vs hypoxia. MTH-1: MutT homolog-1; qRT-PCR: Quantitative real-time polymerase chain reaction.