Evaluation of epithelial-mesenchymal transitioned circulating tumor cells in patients with resectable gastric cancer: Relevance to therapy response

Figure 3 RNA-in situ hybridization analysis of epithelial-to-mesenchymal transition markers in circulating tumor cells from patients with gastric cancer. A: Representative images of five types of CTCs isolated from patients with gastric cancer, based on RNA-ISH staining of E (red dots) and M (green dots) markers. Scale bar, 5 μm; B: Quantitation of EMT features in CTCs based on E and M RNA-ISH staining of histological subtypes of gastric cancer (SRCC and Adenocarcinoma), along with molecular classification of primary tumors (Her2^- and Her2⁺). CTC numbers per 10 mL of blood are listed below. RNA-ISH: RNA-in situ hybridization; CTCs: Circulating tumor cells; E: Epithelial; M: Mesenchymal; EMT: Epithelial-to-mesenchymal transition; DAPI: 4’,6-diamidino-2-phenylindole.