Effects of Ligustrum robustum on gut microbes and obesity in rats

Figure 5 Similarity of gut microbiota compared with the obese group. A: The Jaccard similarity index of T-RFs in the HFD group and the other groups; B: Correlation of body weight with average similarity. Normal: Normal chow diet-fed mice; HFD: High-fat diet-fed control mice; LR-L/M/H: Low-, medium- and high-dose of Ligustrum robustum aqueous extract-treated HFD rats; Hae III (Hha I)-FAM (HEX): Purified PCR products digested by the restriction enzyme Hae III (Hha I) with FAM (HEX)-labeled DNA fragments separated by capillary electrophoresis; n = 10 for LR-L, LR-M, LR-H; n = 9 for Normal and HFD.