Association between hepatocellular carcinoma and tumor necrosis factor alpha polymorphisms in South Korea

Table 1 Used primer and restriction enzyme at each polymorphism

Polymorphisms	Forward primer	Reverse primer	Restriction enzyme1
-238 G>A	5’-AGA AGA CCC CCC TCG GAA CC2-3’	5’-ATC TGG AGG AAG CGG TAG TG-3'	MspI
-308 G>A	5’-AGG CAA TAG GTT TTG AGG GCC AT-3'	5’-TCC TCC CTG CTC CGA TTC CG-3'	NcoI
-857 C>T	5′-AAG TCG AGT ATG GGG ACC CCC CGT TAA-3'	5′-CCC CAG TGT GTG GCC ATA TCT TCT T-3'	AseI
-863 C>A	5'-GGC TCT GAG GAA TGG GTT	5'-CTA CAT GGC CCT GTC TTC GTT ACG-3'	MnlI
-1031 T>C	5’-AGC AAG AGC TGT GGG GAG AA-3'	5’-CCT GTA ACC CAT TCC TCA GAG CC-3'	BbsI

¹All of the restriction enzymes were available from New England Biolabs (MA, United States) and we used the reaction conditions recommended by the instructions;

²The underlined bases in the primer were mismatched with the wild-type sequence in order to introduce the restriction enzyme site.