Case Report
Copyright ©The Author(s) 2015.
World J Gastroenterol. Dec 7, 2015; 21(45): 12963-12969
Published online Dec 7, 2015. doi: 10.3748/wjg.v21.i45.12963
Figure 2
Figure 2 Caspase positive cells and quantitative gene expression profile of mucosal biopsies before and after anti-interleukin-6R therapy. Pairs of colonic tissue samples taken before (A) and after (B) anti-interleukin (IL)-6R therapy, were stained for caspase and assessed regarding caspase positive cells in 14 high-power fields per slide (C); Histological slides before (D) and after (E) IL-6R inhibition were also stained for caspase and CD4 and quantitatively assessed (F); There was a marked reduction of apoptotic LPMCs and mucosal T cells upon tocilizumab treatment. Nuclei were counterstained with DAPI. Scale bars represent 25 μm. Total RNA was isolated from mucosal biopsies taken before (white) and after (black) anti-IL-6R therapy and reverse transcribed (G). Gene expression profiles were analysed by quantitative PCR and normalized to the housekeeping-gene HPRT. There was a marked induction in the expression levels of pro-inflammatory cytokines upon anti-IL-6R treatment.