Heat shock pretreatment improves stem cell repair following ischemia-reperfusion injury via autophagy

Figure 4 Inhibition of autophagy abrogated the effects of heat shock pretreatment on apoptosis reduction in mesenchymal stem cells exposed to H₂O₂ treatment. A: Flow cytometry indicated that 3-MA significantly increased the apoptotic rate in heat shock pretreatment (HSP)-treated mesenchymal stem cells; B: Western blot showed that 3-MA significantly increased Bax and cytochrome C expression, and decreased Bcl-2, LC3-II and beclin 1 expression. The data represent the results of three separate experiments. ^aP < 0.05, ^bP < 0.01 compared with the control group; ^dP < 0.01 compared with the HSP_2h group.