Chemosensitization of HepG2 cells by suppression of NF-κB/p65 gene transcription with specific-siRNA

Figure 4 Expression of nuclear factor-κB or nuclear factor-κB/p65 mRNA in HepG2 cells before and after siRNA transfection. A: The expression of nuclear factor (NF)-κB/p65 mRNA was higher in HepG2 cells than LO2 cells before siRNA transfection. The fragments (145 bp) of NF-κB/p65 mRNA were amplified by reverse transcription polymerase chain reaction, separated on 2% agarose gel, and stained with ethidium bromide; B: Immunohistochemical staining with anti-NF-κB/p65 (streptavidin-peroxidase, original magnification × 40); NF-κB/p65 positive material was a brown-yellow fine particle layer and localized in the cytoplasm and nucleus of HepG2 cells (L); the expression of NF-κB/p65 positive material in the cytoplasm and nucleus of HepG2 cells with siRNA transfection (R); C: Western-blotting of NF-κB/p65 in cytoplasm and nucleus of HepG2 cells with siRNA transfection. Lanes 1, cytoplasm of HepG2 cells; Lanes 2, nucleus of HepG2 cells; Lanes 3, cytoplasm of HepG2 cells with 100 nmol/L of siRNA transfection; and Lanes 4, nucleus of HepG2 cells with 100 nmol/L of siRNA transfection. p65, 65 kDa; β-actin, 42 kDa, as the control protein.