Chemosensitization of HepG2 cells by suppression of NF-κB/p65 gene transcription with specific-siRNA

Figure 2 Expressions of nuclear factor-κB/p65, P-p65, and P-gp induced by different doses of doxorubicin in HepG2 cells. Lane 1, control without doxorubicin; Lane 2, 0.5 μmol/L of doxorubicin for 4 h; Lane 3, 0.5 μmol/L of doxorubicin for 24 h; Lane 4, 1.0 μmol/L of doxorubicin for 4 h; and Lane 5, 1.0 μmol/L of doxorubicin for 24 h. A: The expressions of NF-κB/p65 and P-p65 in HepG2 cells with different doses of doxorubicin were analyzed by western blotting. NF-κB/p65 and P-p65 shows a 65 kDa band, and β-actin shows a 42 kDa band as control; B: The relative levels of P-glycoprotein (P-gp) expression to β-actin in HepG2 cells with different doses of doxorubicin at different times, data are means of independent triplicate experiments; C: the analysis of P-gp expression in HepG2 cells with different doses of doxorubicin by western blotting. P-gp, 170 kDa; β-actin, 42 kDa as control protein. The action time of HepG2 cells with different doses of doxorubicin.