Peroxisome proliferator-activated receptor-delta agonist ameliorated inflammasome activation in nonalcoholic fatty liver disease

Figure 7 Effects of GW501516 on reactive oxygen species production and associated molecular pattern-activated protein kinase-α phosphorylation in HepG2 cells. Intracellular ROS production was quantified using the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) (A). GW501516 inhibited lipopolysaccharide (LPS)- and palmitic acid (PA)-induced ROS generation. Representative immunoblot and graphic presentation of p-AMPK-α and total AMPK-α protein levels in HepG2 cells are shown (B). GW501516 tended to increase the phosphorylation of AMPK-α in PA- and LPS-treated cells. Data are expressed as the mean ± SD (5-6 mice per group). ^aP < 0.05 vs control cells, ^cP < 0.05 vs PA + LPS-treated cells.