Peroxisome proliferator-activated receptor-delta agonist ameliorated inflammasome activation in nonalcoholic fatty liver disease

Figure 4 Effects of GW501516 on palmitic acid- and lipopolysaccharide-induced inflammasome and pro-inflammatory cytokine in HepG2 cells. Relative mRNA levels of NLRP3 (A), NLRP6 (B), NLRP10 (C), caspase-1 (D), and IL-1β (E) in HepG2 cells were analyzed by RT-PCR. GW501516 inhibited PA- and LPS-induced mRNA expression of several inflammasome components, caspase-1, and IL-1β. Representative immunoblot and graphic presentation show protein levels of caspase-1 and IL-1β (F) in HepG2 cells. PA and LPS tended to elicit caspase-1 and IL-1β release, and GW501516 reduced this processing significantly. Data are expressed as the mean ± SD. ^aP < 0.05 vs control cells, ^cP < 0.05 and ^bP < 0.01 vs PA + LPS-treated cells.