Retinoic acid receptor α promotes autophagy to alleviate liver ischemia and reperfusion injury

Figure 4 Retinoic acid receptor α inhibits reactive oxygen species-induced apoptosis in vitro. LE540, an inhibitor of RARα, was cocultured with FL83B cells for 24 h, and then the cells were harvested 6 h after H₂O₂ treatment. A: Expression levels of Bcl-2, cleaved caspase 3 and β-actin were measured by Western blot; B: The number of apoptotic cells was assessed by AV and PI labeling. Mean ± SD, n = 3-5/group, ^aP < 0.05 vs the DMSO group; ^bP < 0.05 vs the H₂O₂ group. RARα: Retinoic acid receptor α; AV: Annexin V; PI: Propidium iodide; FITC: Fluorescein isothiocyanate; ATRA: All-trans retinoic acid.