Hepatitis C virus NS5A promotes insulin resistance through IRS-1 serine phosphorylation and increased gluconeogenesis

Figure 1 Hepatitis C virus infection modulates insulin signaling in hepatitis C virus infected human hepatoma cells. Cellular lysates were made from mock and hepatitis C virus (HCV) infected Huh7 and Huh7.5 cells. An equal amount of cellular lysates were subjected to Western blot assay using p-IRS-1 Ser³⁰⁷and p-Akt Ser⁴⁷³. HCV NS3 protein expression represents the level of HCV infection. Cellular actin was used as an internal control to verify protein loading in each lane. IRS: Insulin receptor substrate; NS: Nonstructural protein.