Zebrafish as a disease model for studying human hepatocellular carcinoma

Table 3 Zebrafish animal models of liver disease and hepatocellular carcinoma

Transgene name	Expression system	Liver pathology	Ref.
cnr1 (Zebrafish)	Tet-off-inducible	Steatosis	[132]
edn1 (Zebrafish)	Constitutive	Steatosis, bile duct dilation, hyperplasia and HCC	[69]
gankyrin (Zebrafish)	Constitutive	Atrophy, hypoplasia and steatosis	[123]
HBx (Human)	Constitutive	Hypoplasia and steatosis	[120]
HBx + AFB1 (Human)	Constitutive	Hepatitis, steatosis and hyperplasia	[134]
HBx + HCV (Human)	Tet-off-inducible	Intrahepatic cholangiocarcinoma	[81]
HBx + p53M214 (Human)	Constitutive	Chronic inflammation, steatosis, bile duct dilation, dysplasia and HCC	[70]
HBx + src (Human/Zebrafish)	Constitutive	Chronic inflammation, steatosis, bile duct dilation, dysplasia and HCC	[70]
HCV (Human)	Constitutive	Steatosis	[29]
HCV + TAA (Human)	Constitutive	Steatosis and HCC	[29]
kras-G12V (Zebrafish)	Mifepristone	Hyperplasia and HCC	[82]
kras-G12V (Zebrafish)	Constitutive	Hyperplasia and hepatocellular adenoma	[82]
kras-G12V (Zebrafish)	Tet-on-inducible	Hyperplasia, hepatocellular adenoma and HCC	[137]
kras-G12V + p53M214 (Zebrafish)	Constitutive	Hyperplasia and hepatocellular adenoma	[82]
kras-G12V + RhoA (Zebrafish)	Tet-on-inducible	Hyperplasia, hepatocellular adenoma and HCC	[137]
kras-G12V + RhoAG14V (Zebrafish)	Tet-on-inducible	Hyperplasia, hepatocellular adenoma and HCC	[137]
kras-G12V + RhoAT19N (Zebrafish)	Tet-on-inducible	HCC	[137]
Lc3 (Rat)	Constitutive	Investigation of liver autophagy	[141]
mdm2 (Zebrafish)	Constitutive	Atrophy, contraction and hypoplasia	[121]
MYC (Mouse)	Tet-on-inducible	Hyperplasia and hepatocellular adenoma	[83]
myca (Zebrafish)	Mifepristone	Small, typical, hypervascular and ascites of liver tumor	[143]
myca + p53M214 (Zebrafish)	Mifepristone	Small, typical, hypervascular and ascites of liver tumor	[143]
mycb (Zebrafish)	Mifepristone	Small, typical, hypervascular and ascites of liver tumor	[143]
orf A (Human)	GAL4/UAS	Delayed onset of liver tumor	[79]
src (Zebrafish)	Constitutive	Chronic inflammation, steatosis, bile duct dilation, hyperplasia, dysplasia and HCC	[70]
src + p53M214 (Zebrafish)	Constitutive	Steatosis, hyperplasia, dysplasia and HCC	[70]
UHRF1 (Human)	Constitutive	Atypical cells, dysplastic foci and HCC	[124]
UHRF1 + p53M214 (Human)	Constitutive	Atypical cells, dysplastic foci and HCC	[124]
xmrk (Xiphophorus)	Tet-on-inducible	Hyperplasia, hepatocellular adenoma and HCC	[80]
yy1 (Zebrafish)	Constitutive	Steatosis	[131]
zfBLP1 (Zebrafish)	Constitutive	Hyperplasia	[133]
zfMcl-1α (Zebrafish)	Constitutive	Hyperplasia	[133]

HCC: Hepatocellular carcinoma; HBx: Hepatitis B virus X protein; HCV: Hepatitis C virus.