Weichang’an and 5-fluorouracil suppresses colorectal cancer in a mouse model

Figure 4 Immunohistochemistry detection of β-catenin and matrix metalloproteinase-7 in orthotopic tumors and hepatic metastatic tumors. A, B: Integrated optical density (OD) of β-catenin and matrix metalloproteinase (MMP)-7 protein expressed in orthotopic tumors (A) or hepatic metastatic tumors (B) from mice in the CON, WCA, 5-FU and WCA + 5-FU groups. Data are presented as mean ± SD and ^aP < 0.05 vs corresponding CON group; C: IHC staining for β-catenin in orthotopic tumors (magnification × 200); D: IHC staining for MMP-7 in orthotopic tumors (magnification × 200); E: IHC staining for β-catenin in hepatic metastatic tumors (magnification × 200); F: IHC staining for MMP-7 in hepatic metastatic tumors (magnification × 200). WCA: Weichang’an; 5-FU: 5-fluorouracil; CON: Control.