Equol inhibits proliferation of human gastric carcinoma cells via modulating Akt pathway

Figure 4 Apoptotic effect of equol on MGC-803 cells. A: Cells were treated with different concentrations of equol for 48 h, then subjected to Annexin V/PI apoptosis assay as described in “Materials and methods”; B: The effect of equol on the expression of end point markers of apoptosis. Cells were treated with 20 μmol/L of equol for different durations. Then, cells were harvested, and the expression of cleaved PARP and caspase-3 was determined by Western blot analysis. GAPDH antibody was utilized as a loading control (^aP < 0.05 vs control; ^bP < 0.01 vs control).