Ursodeoxycholic acid induces apoptosis in hepatocellular carcinoma xenografts in mice

Figure 4 Expression of cleaved-caspases 3 and 9 is induced in response to ursodeoxycholic acid in xenografts. Western blot analysis was performed with protein lysates prepared from control and treated tumors. Blots were incubated with antibody against cleaved-caspases 3 and 9, and β-actin was used as an internal control. 0: Control; lanes 1-3: Ursodeoxycholic acid (UDCA) 30 mg/kg per day, UDCA 50 mg/kg per day, and UDCA 70 mg/kg per day.