Ursodeoxycholic acid induces apoptosis in hepatocellular carcinoma xenografts in mice

Figure 3 Systemic administration of ursodeoxycholic acid induces apoptosis in BEL7402 xenografts in immunodeficient mice. A: TUNEL staining performed to detect apoptotic cells in situ in tissue sections prepared from control and treated xenografts as indicated (magnification × 400); B: Apoptotic cell numbers from control and treated xenografts represented graphically. Data are presented as the mean ± SD for 1000 cells in randomly selected fields on three sections for each group. Differences between control and treated xenografts reached statistical significance (30 mg/kg per day, ^aP < 0.05; 50 mg/kg per day, ^eP < 0.05; 70 mg/kg per day, ^fP < 0.01).