TP53 codon 72 Arg/Arg polymorphism is associated with a higher risk for inflammatory bowel disease development

Figure 1 Polymerase chain reaction-restriction fragment length polymorphisms analysis. A: A representative analysis of the polymerase chain reaction (PCR) product. The product corresponded to TP53 with a single band at 353; B: The PCR-restriction fragment length polymorphisms (PCR-RFLP) analysis and sequencing results of TP53 codon 72 polymorphism. The Pro allele was not cleaved by BstUI and had a single 353 bp band (lanes 1 and 2). The Arg allele was cleaved by BstUI, yielding two fragments 213 and 140 bp (lanes 5 and 7). Heterozygotes contained all three bands (lanes 3, 4 and 6). RFLP results were confirmed by direct sequencing of 100 randomly selected samples. M: Molecular weight marker.