IGF-1 promotes the growth and metastasis of hepatocellular carcinoma via the inhibition of proteasome-mediated cathepsin B degradation

Figure 5 PA28γ overexpression inhibited IGF-1-induced cancer cell growth and invasion. A: Hep1-6 cells infected with lentivirus PA28γ were treated with IGF-1 (100 nmol/L). MTT assay was detected on these cells; B: Wound healing assay of Hepa 1-6 cells overexpressing PA28γ treated with IGF-1 for 24 h; C: PA28γ overexpression inhibited IGF-1-induced cancer cell invasion. Hepa 1-6 cells were infected with lentivirus containing PA28γ. After 24 h, the cells were treated with IGF-1 for 12 h. The data are presented as a representative Transwell assay (scale bar, 20 μm). The data are presented as the mean ± SE of 3 assays. Student’s t-test. ^aP < 0.05; ^bP < 0.01.