Natural evolution of hepatitis C virus infection in hemodialysis Tunisian patients and CTLA-4 SNP's

Table 1 Sequences of primer and PCR conditions

Polymorphisms	Primers	Temperature, time and cycles for PCR
CTLA-4(+49) A/G	Forward:	Initial denaturation for 4min at 94 °C, 35 cycles of 30 s at 94 °C, 30 s at 67 °C and 1 min at 72 °C and final elongation 5 min at 72 °C
	5’CAAGGCTCAGCTGAACCTGGGT3’
	Reverse:
	5’TACCTTTAACTTCTGGCTTTG3’
CTLA-4(+6230) G/A	Forward	Initial denaturation for 5 min at 94 °C, 30 cycles of 40 s at 94 °C, 30 s at 61 °C and 50 s at 72 °C and final elongation 7 min at 72 °C
	CT60 Sens:
	5’ CACCACTATTTGGGATATACC 3’
	Reverse
	CT60 Antis:
	5’ AGCTCTATATTTCAGGAAGGC 3’