Mechanism of QHF-cisplatin against hepatocellular carcinoma in a mouse model

Table 1 Configuration of the 12% separating gel and 5% stacking gel

Component	12% separating gel	5% stacking gel
	7.5 mL	3 mL
H2O	2.47 mL	2.1 mL
1.5 mol/L Tris (pH 8.8)	1.88 mL	-
1.0 mol/L Tris (pH 6.8)	-	0.375 mL
30% Ace-Bis (29:1)	3 mL	0.5 mL
10% SDS	75 μL	30 μL
10% APS	75 μL	30 μL
TEMED	3 μL	3 μL

TEMED: N,N,N’,N’-tetramethylethylendiamine; SDS: Sodium dodecyl sulfate; APS: Ammonium persulfate.