Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury

doi:10.3748/wjg.v21.i35.10091

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 21, 2015; 21(35): 10091-10103
Published online Sep 21, 2015. doi: 10.3748/wjg.v21.i35.10091

Table 2 Primers used for real-time polymerase chain reaction analysis

Gene symbol	Sequence (5'→3')
Rpl10a	Forward	GAAGAAGGTGCTGTGTTTGGC
Rpl10a	Reverse	TCGGTCATCTTCACGTGGC
Arbp	Forward	GGCGACCTGGAAGTCCAACTA
Arbp	Reverse	CATTGTCTGCTCCCACAATGAA
Rpl15	Forward	GCTTTAGTAGCAGCTGGTGTGTGA
Rpl15	Reverse	ACCCAAGACGAATTGATTGGAA
Rps16	Forward	TCCAAGGGTCCGCTGCAGTC
Rps16	Reverse	CGTTCACCTTGATGAGCCCATT
Rpl3	Forward	TGTATTGGAGCTTGGCATCCTG
Rpl3	Reverse	ACCATCCTTGATGAGGTAGCCTTG
Rps18	Forward	AAGTTTCAGCACATCCAGCGAGTA
Rps18	Reverse	TTGGTGAGGTCAATGTCTGCTTTC
Rps3	Forward	GATCATGTGAGCATTGTGGAACCTA
Rps3	Reverse	CTCCAGATGCAGCTGCCAAG
Cyp1a2	Forward	TCAACCTCGTGAAGAGCAGCA
Cyp1a2	Reverse	GTCCTGGATACTGTTCTTGTTGAAGTC
Gstm1	Forward	TTCGTGCAGACATTGTGGAGA
Gstm1	Reverse	CTTGCCCAGGAACTCAGAGTAGA
Gclc	Forward	GTGGACACCCGATGCAGTATTC
Gclc	Reverse	CATCCACCTGGCAACAGTCATTAG
Gsta3	Forward	AATAGGCTGAGCAGGGCTGATG
Gsta3	Reverse	GGTTGCTGACTCTGGTTCTCAGG
Gstt3	Forward	ATGCCTTTGCCCAGGTGAAC
Gstt3	Reverse	GTGTGCTGCCAAGCCAGGTA
Gstp1	Forward	GAGGACCTTCGATGCAAATATGGTA
Gstp1	Reverse	CTGGGACAGCAGGGTCTCAA

Citation: Yoshida K, Ushida Y, Ishijima T, Suganuma H, Inakuma T, Yajima N, Abe K, Nakai Y. Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury. World J Gastroenterol 2015; 21(35): 10091-10103
URL: https://www.wjgnet.com/1007-9327/full/v21/i35/10091.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i35.10091