β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation

Figure 8 IPA-3 pretreatment increased radiation-induced cell death in gastric cancer cells. Cells were seeded into 6-well plates and exposed to the indicated concentrations of IPA-3 pretreatment. 24 h after 8 Gy IR, cells were collected, incubated with annexin V-FITC and PI, and analyzed using FCM. The experiment was performed in triplicate. Representative FCM results are shown. A: MKN45 cell line; B: SGC7901 cell line. PI: Propidium iodide; IR: Ionizing radiation; FCM: Flow cytometry.