β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation

Figure 3 β-elemene pretreatment increased radiation-induced cell death in gastric cancer cells. Cells were seeded into 6-well plates, incubated overnight, and exposed to the indicated concentration of β-elemene pretreatment prior to IR. 24 h after 8 Gy IR, cells were collected, incubated with annexin V-FITC and PI, and analyzed using FCM. Examples of independent FCM results are shown. A: MKN45 cell line; B: SGC7901 cell line. FCM: Flow cytometry. PI: Propidium iodide; IR: Ionizing radiation.