β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation

Figure 2 β-elemene pretreatment decreases clonogenic survival following ionizing radiation in gastric cancer cells. Cells were seeded into 6-well plates as indicated. After 4 to 6 h attachment, cells were treated with or without β-elemene for 18 h, followed by exposure to the indicated doses of IR. Then, cells were incubated as described above and colonies were counted. Cell survival curves were fitted using Graphpad Prism 5.1 software according to the multi-target single hit model. The results are representative of duplicate independent experiments. A: MKN45 cell line; B: SGC7901 cell line; Dots and bars: mean ± SD. IR: Ionizing radiation.