Macrophage migration inhibitory factor as a potential prognostic factor in gastric cancer

Figure 3 Macrophage migration inhibitory factor mRNA expression in AGS cells. A: Electrophoresis with 2% agarose gel and real-time polymerase chain reaction analysis show substantially reduced macrophage migration inhibitory factor (MIF) mRNA levels compared with the GAPDH internal control; B: Gray values in AGS cells transfected with MIF small-interfering RNA pairs (si-1 or si-2) or scrambled small-interfering RNA (negative control). Results are representative of three independent experiments for each AGS and MKN-45 cells. NC: Negative control.