Notch1 downregulation combined with interleukin-24 inhibits invasion and migration of hepatocellular carcinoma cells

Figure 2 γ-secretase inhibitor-I/interleukin-24 combined treatment induces apoptotic cell death in hepatocellular carcinoma cells. A: Apoptotic effects induced by treatment of HepG2 cells with GSI-I and/or IL-24 for 24 h. Apoptotic morphology was evaluated by staining of the cells with Hoechst 33258 (magnification × 200); B: Annexin V positive cells were quantified by flow cytometric analysis after double staining of cells with Annexin V and propidium iodide at 6 h of treatment. Results are representative of three independent experiments. GSI-I: γ-secretase inhibitor-I; IL: Interleukin.