Establishment of a hepatic cirrhosis and portal hypertension model by hepatic arterial perfusion with 80% alcohol

Figure 5 Pathological variation. A: Within 6 h after perfusion in experimental group 2, thrombosis was evident in interlobular veins; B: At 2 wk after perfusion in experimental group 2, irregular, massive hepatocyte degeneration and necrosis were observed; C: At 4 wk after perfusion in experimental group 2, bile duct proliferation and dilation were evident; D: At 6 wk after perfusion in experimental group 2, pseudo-lobules formed with fibrous tissue proliferation and bridging fibrosis. Hematoxylin-eosin staining, magnification × 40 (A); × 200 (B-D).