Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication

Table 1 Sequences and locations of hepatitis B virus-specific gRNAs in the hepatitis B virus genome of genotypes A-D

gRNA No.	Nucleotide position	Sequence (GN18-20NGG, 5’-3’)	Genotype
1	56-75	CCTGCTGGTGGCTCCAGTTC	A/B/C/D
2	182-200	GGACCCCTGCTCGTGTTAC	A/B/C/D
3	415-433	GCTGCTATGCCTCATCTTC	A/B/C/D
4	640-658	ATGGGAGTGGGCCTCAGTC	A/B/C
5	1179-1197	AGTGTTTGCTGACGCAACC	A/B/C/D
6	1393-1410	GCCAACTGGATCCTGCGC	B/C/D
7	1521-1540	GGGGCGCACCTCTCTTTACG	A/B/C/D
8	1578-1597	GAGGTGAAGCGAAGTGCACA	A/B/C/D
9	1589-1608	CTTCACCTCTGCACGTCGCA	B/C/D
10	1775-1794	AGGAGGCTGTAGGCATAAAT	A/B/C/D
11	1859-1878	AGCTTGGAGGCTTGAACAGT	A/B/C/D
12	1865-1884	CAAGCCTCCAAGCTGTGCCT	A/B/C/D
13	2336-2355	ACTACTGTTGTTAGACGACG	C/D
14	2367-2386	CGAGGGAGTTCTTCTTCTAG	A/B/C/D
15	2390-2409	GATTGAGACCTTCGTCTGCG	B/C/D