MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer

Figure 4 miR-30b decreases eukaryotic translation initiation factor 5A2 expression by targeting its 3’-UTR. A: The binding site of miR-30b in the 3’-UTR of eukaryotic translation initiation factor 5A2 (EIF5A2) is highly conserved across species; B: The putative binding sites for miR-30b was found in the 3’-UTR of EIF5A2 at 3664-3671bp; C: miR-30b mimic downregulated luciferase activities controlled by wild-type 3’-UTR of EIF5A2, but did not affect luciferase activity controlled by mutant 3’-UTR of EIF5A2; D: The expression levels of EIF5A2 protein in different gastric cell lines; E: The expression levels of EIF5A2 and of its downstream genes were detected by Western blot analysis in AGS and MGC803 cells transfected with the miR-30b mimic or control for 48 h. β-actin was used as an internal control; F: Western blot analysis of EIF5A2 and its downstream genes transfection of miR-30b inhibitor in AGS and MGC803 cells.