Influence of the hTERT rs2736100 polymorphism on telomere length in gastric cancer

Figure 2 Association of the rs2736100 polymorphism with hTERT mRNA expression in 5 gastric cancer cell lines. A: The genotype of rs2736100 polymorphism of the hTERT gene was determined by polymerase chain reaction-restriction fragment length polymorphism. The genotype of AGS and MKN1 cells was C/C homozygote, MKN45 and SNU-638 was heterozygote, and KATO-III was A/A homozygote. GKN1 treatment significantly reduced the expression of hTERT gene in 5 gastric cancer cell lines (^aP < 0.05); B: The genotype of rs2736100 polymorphism of the hTERT gene was closely associated with hTERT mRNA expression in non-treated gastric cancer cells; C: Fold changes of hTERT mRNA expression in GKN1-treated compared to non-treated gastric cancer cells were assessed by real time QPCR. GKN1 treatment markedly down-regulated hTERT mRNA expression, especially in MKN-45 and SNU-638 cells with C/A heterozygote and KATO-III cell with A/A homozygote. hTERT: Human telomerase reverse transcriptase.