Mechanisms of pyruvate kinase M2 isoform inhibits cell motility in hepatocellular carcinoma cells

Figure 2 Knockdown of pyruvate kinase M2 isoform promoted the migration and invasion of HepG2 and Huh-7 cells upon epidermal growth factor stimulation. A: A cross-shaped wound was created in the monolayer, and stably transfected HepG2 and Huh-7 cells were cultured for an additional 24 h with epidermal growth factor (EGF) (50 ng/mL). Representative images of the wounded region are shown; B: The results of the migration assay are also shown as graphs (^aP < 0.05); C: The invasion potential of the HepG2 and Huh-7 stable cells was assessed using the BD transwell chamber assay with 50 ng/mL EGF in the lower chamber for 36 h. The cells that migrated to the lower side of the filter were stained, photographed, and counted; D: The data are expressed as the mean ± SD from three independent experiments, ^aP < 0.05 between groups.