NOB1 is essential for the survival of RKO colorectal cancer cells

Figure 4 Suppression of tumor growth by lentivirus-mediated NOB1-siRNA in RKO cell xenograft mouse model. A: RKO cell xenograft mice were injected intratumorally with lentivirus-mediated NOB1-siRNA, scr-siRNA, or PBS. The size of the primary tumors was measured every week. Mice were sacrificed after 5 wk. NOB1-siRNA reduced NOB-1 expression in xenografted tumor tissue as determined by Western blot; B: NOB1-siRNA induced significant apoptosis in xenografted tumor tissue detected by TUNEL staining as shown in representative images; C: Plotted percentages of apoptotic cells; D: Typical pictures from RKO cell xenografted tumors infected by PBS, src-RNA and NOB1-siRNA; E: Plotted tumor weight; F: Tumor volume in RKO cell xenograft mouse model after NOB1-siRNA infection. ^aP < 0.05 vs scr-siRNA. scr-siRNA: Cells infected with lentivirus-mediated scramble small interfering RNA; NOB1-siRNA: Cells infected with lentivirus-mediated NOB1-siRNA; PBS: Phosphate buffered saline.