NOB1 is essential for the survival of RKO colorectal cancer cells

Figure 2 Effect of NOB1 knockdown on cell growth in RKO cells. A: The infected cells expressing GFP were imaged and counted using the Cellomics ArrayScan^TM High Content Screening (HCS) Reader once a day for 5 d. Cell growth curves in scr-siRNA and NOB1-siRNA infected RKO cells are shown; B: Cell growth was detected by the colony formation assay in RKO cells 14 d after infection of scr-siRNA and NOB1-siRNA cells. RKO cells were seeded at 500 cells/well and allowed to form colonies. Cell colonies were imaged and counted using the Cellomics ArrayScan^TM HCS Reader. Data are presented as mean ± SD of three independent experiments. ^aP < 0.05 vs scr-siRNA. scr-siRNA: Cells infected with lentivirus-mediated scramble small interfering RNA; NOB1-siRNA: Cells infected with lentivirus-mediated NOB1-siRNA; GFP: Green fluorescent protein; qRT-PCR: Quantitative reverse transcription polymerase chain reaction.